Open AccessSatellite DNA III and alkaline Giemsa staining
Author(s) -
Erica M. Bühler,
T. Tsuchimoto,
Linda P. Jurik,
G Stalder
Publication year - 1975
Publication title -
human genetics
Language(s) - English
Resource type - Journals
eISSN - 1432-1203
pISSN - 0340-6717
DOI - 10.1007/bf00285384
Subject(s) - giemsa stain , staining , secondary constriction , biology , centromere , satellite dna , chromosome , satellite , microbiology and biotechnology , dna , g banding , genetics , karyotype , gene , engineering , aerospace engineering
Satellite DNA III visualized by staining chromosomes with Giemsa at pH 10-12. Evidence is presented that besides the secondary constriction of chromosome 9, satellite III contained in considerable amount in the long arms of chromosome 20, giving rise to a clearly visible secondary constriction just below the centromere. The latter finding confirms that reported by Bobrow et al. (1972). The long arms of the Y chromosome also show strong staining with alkaline Giemsa, the region of staining corresponding exactly with the intensely flourescing area. This is interpreted as possible evidence for the presence of satellite DNA III in the distal long arms of the human Y chromosome.