Characterization of the cleavage site and the recognition sequence of the I-CreI DNA endonuclease encoded by the chloroplast ribosomal intron of Chlamydomonns reinhardtii | Zendy

Franz Dürrenberger | Zendy; JeanDavid Rochaix | Zendy

Open Access

Characterization of the cleavage site and the recognition sequence of the I-CreI DNA endonuclease encoded by the chloroplast ribosomal intron of Chlamydomonns reinhardtii

Author(s) -

Franz Dürrenberger,

JeanDavid Rochaix

Publication year - 1993

Publication title -

mgg molecular and general genetics

Language(s) - English

Resource type - Journals

eISSN - 1432-1874

pISSN - 0026-8925

DOI - 10.1007/bf00277141

Subject(s) - biology , intron , homing endonuclease , chlamydomonas reinhardtii , genetics , endonuclease , recognition sequence , ribosomal rna , dna , ribosomal dna , group i catalytic intron , cleavage (geology) , gene , computational biology , restriction enzyme , rna , rna splicing , phylogenetics , mutant , paleontology , fracture (geology)

The chloroplast ribosomal intron of Chlamydomonas reinhardtii encodes a sequence-specific DNA endonuclease (I-CreI), which is most probably involved in the mobility of this intron. Here we show that I-CreI generates a 4 bp staggered cleavage just downstream of the intron insertion site. The I-CreI recognition sequence is 19-24 bp in size and is located asymmetrically around the intron insertion site. Screening of natural variants of the I-CreI recognition sequence indicates that the I-CreI endonuclease tolerates single and even multiple base changes within its recognition sequence.

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