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EFFECTS OF TRANSFORMING GROWTH FACTOR‐β 1 AND FIBRONECTIN ON SPARC EXPRESSION IN CULTURES OF HUMAN PERIODONTAL LIGAMENT CELLS
Author(s) -
Fujita Tsuyoshi,
Shiba Hideki,
Sakata Masatoshi,
Uchida Yuushi,
Ogawa Tetsuji,
Kurihara Hidemi
Publication year - 2002
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.2002.0966
Subject(s) - fibronectin , extracellular matrix , osteonectin , periodontal fiber , transforming growth factor , chemistry , transforming growth factor beta , cell culture , microbiology and biotechnology , messenger rna , gene expression , extracellular , biology , biochemistry , gene , enzyme , medicine , dentistry , alkaline phosphatase , genetics , osteocalcin
Transforming growth factor‐β 1 (TGF‐β 1 ) increases synthesis of secreted protein, acidic and rich in cysteine (SPARC), as well as fibronectin (FN) and type I collagen. However, little is known about the regulatory mechanism of SPARC expression. We examined the effect of FN on SPARC expression by TGF‐β 1 in cultures of human periodontal ligament cells (HPL cells). TGF‐β 1 increased the SPARC and SPARC mRNA levels in HPL cells. Extracellular matrix (ECM) produced by HPL cells in the presence of TGF‐β 1 also increased the SPARC levels. Contents of FN and type I collagen in the ECM were increased by TGF‐β 1 . HPL cells cultured on FN‐coated plates secreted more SPARC than those on non‐coated plates. However, type I collagen had little effect on SPARC levels. The addition of anti‐α5 antibody to the cultures abolished the increase in SPARC mRNA expression by TGF‐β 1 . This study demonstrated that FN may be partly involved in the increase in SPARC expression by TGF‐β 1 in HPL cells.