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MONOCYTES MODULATE THE IN VITRO BASAL FREQUENCY OF SISTER CHROMATID EXCHANGES OF PLASMA LEUKOCYTE CULTURES AND MITOTIC ACTIVITY OF SUPPRESSOR‐CYTOTOXIC T8 HUMAN LYMPHOCYTES
Author(s) -
Soloneski Sonia M.,
Reigosa Miguel A.,
Garcia Carlos F.,
Larramendy Marcelo L.
Publication year - 2002
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.2002.0931
Subject(s) - in vitro , kinetics , sister chromatids , lymphocyte , mitosis , cytotoxic t cell , biology , microbiology and biotechnology , basal (medicine) , chemistry , immunology , biochemistry , endocrinology , gene , insulin , chromosome , physics , quantum mechanics
The effect of monocytes (MNs) on baseline SCEs and kinetics of human lymphocytes in plasma leukocyte (PLCs) and whole blood cultures (WBCs) was studied. Baseline SCEs in PLCs were nearly two‐fold over WBCs. No differences in SCEs were observed between PLCs and MN‐depleted PLCs, indicating that SCEs from PLCs are independent of MNs. MNs titration into PLCs decreased proportionally SCEs. Reconstitution of depleted PLCs with concentration of MNs equivalent or higher than those of PLC decreased SCEs. No variations of lymphocyte kinetics in PLCs were observed in the absence/presence of MNs. The proportion of B and T‐cell subsets among interphasic lymphocytes were similar in PLC in the absence/presence of MNs, but a significant increase in the proportion of mitotic T8 lymphocytes was observed. Accordingly, MNs modulate both the in vitro basal SCEs and the mitotic activity of T8, but not their cell‐cycle kinetics.