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SULPHATE INFLUX IN THE ERYTHROCYTES OF NORMOTENSIVE, DIABETIC AND HYPERTENSIVE PATIENTS
Author(s) -
Romano L.,
Scuteri A.,
Gugliotta T.,
Romano P.,
Luca G.,
Sidoti A.,
Amato A.
Publication year - 2002
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.2002.0874
Subject(s) - medicine , nifedipine , endocrinology , diabetes mellitus , potassium , sodium , calcium , magnesium , intracellular , essential hypertension , chemistry , pathophysiology , antagonist , blood pressure , biochemistry , receptor , organic chemistry
This study aimed to show that modifications in intracellular metabolism are implicated in the pathophysiology of diabetes mellitus and essential hypertension. In fact, total magnesium, calcium, sodium and potassium concentrations, measured in the erythrocytes of normotensive, diabetic and hypertensive patients, have given the following results: a lower intracellular potassium concentration in the erythrocytes of diabetic and hypertensive patients than the erythrocytes of normotensive patients and a more elevated sodium, magnesium, calcium concentrations in the erythrocytes of diabetic and hypertensive patients than the normotensive. Because of the importance of Mg 2+ and Ca 2+ in metabolic enzyme regulation and their interaction with both Hb and band 3 protein, we examined SO2−4 kinetic influx in the erythrocytes of normotensive, hypertensive and diabetic patients. The kinetic plots showed different profiles over the three groups and the fluxes were found to be 0.024, 0.061 and 0.072mmol×(l cells×min) −1 in normotensive, hypertensive and diabetic patients, respectively. We also found that the Vmax and Km of sulphate influx, obtained by Hofstee plots, increased in the erythrocytes of hypertensive and diabetic patients compared with control cells. In contrast, sulphate influx in the erythrocytes of diabetic and hypertensive patients in the presence of Nifedipine, a calcium antagonist, showed no difference either in the rate constants or in the kinetic profiles, compared to the normotensive control subjects.