Premium
CHANGES IN FUSED CELLS INDUCED BY HVJ (SENDAI VIRUS): REDISTRIBUTION OF CYTOPLASMIC ORGANELLES AND CYTOSKELETAL REORGANIZATION
Author(s) -
Hirayama Etsuko,
Ama Masako,
Takahashi KenIchiro,
Hiraki Akihiro,
Kim Jeman
Publication year - 2002
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.2002.0862
Subject(s) - organelle , cytoskeleton , cytoplasm , sendai virus , microtubule , microbiology and biotechnology , biology , intermediate filament , vimentin , mitochondrion , cell , chemistry , virus , biochemistry , virology , immunology , immunohistochemistry
To understand the relationship between the location of organelles and cellular function, we examined the dynamic state of cytoplasmic organelles and cytoskeleton in polynuclear Ehrlich ascites tumor (EAT) cells fused with hemagglutinating virus of Japan (HVJ; Sendai virus) by confocal laser scanning microscopy. Irregular fused cells gradually became spherical during culture, and nuclei and mitochondria were redistributed in the fused cell; nuclei formed a cluster surrounded by mitochondria. F‐actin, vimentin, and microtubules were also reorganized with the redistribution of cell organelles. Further, when the morphological change was inhibited by L4‐1, a chlorophyll‐like substance derived from silkworm faeces, or pyropheophorbide‐a, the arrangement of organelles and cytoskeleton remained disturbed, suggesting that the movement of the cytoskeleton is closely associated with cell shape and the distribution of cytoplasmic organelles.