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INABILITY OF 50HZ MAGNETIC FIELDS TO REGULATE PKC‐ AND CA 2+ ‐DEPENDENT GENE EXPRESSION IN JURKAT CELLS
Author(s) -
Still Maria,
Lindström Ewa,
Ekstrand A. Jonas,
Mild Kjell Hansson,
Mattsson MatsOlof,
Lundgren Erik
Publication year - 2002
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.2001.0837
Subject(s) - jurkat cells , ionomycin , reporter gene , transfection , luciferase , microbiology and biotechnology , intracellular , protein kinase c , cell culture , enhancer , chemistry , calcium in biology , calcium , inositol , gene expression , biology , kinase , gene , t cell , biochemistry , immunology , receptor , immune system , organic chemistry , genetics
We have previously reported that the T cell line Jurkat registers the exposure of a sinusoidal extremely low frequency magnetic field at the level of the plasma membrane, resulting in activation of the tyrosine kinase p56 lck , increase in inositol‐3‐phosphate levels and increase in intracellular calcium concentration within minutes. To elucidate if these events associated with changes in intracellular calcium ion levels were biologically significant, transient transfections of Jurkat cells were performed with calcium‐ion dependent reporter constructs. Three different enhancer/promoter constructs were studied coupled to the luciferase reporter gene. The luciferase activity of each construct was measured after treatment of transfected cells to EMF exposure alone, or in combination with ionomycin, phorbol ester or cross‐linking anti‐CD3 antibodies. There was no indication that the used EMFs could influence any of these reporter constructs.