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MITOGENIC STIMULATION IN ISOPROTERENOL TREATED CELLS OF DICTYOSTELIUM DISCOIDEUM
Author(s) -
Kumar Akhilesh,
Chatterjee Samar
Publication year - 2002
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.2001.0820
Subject(s) - dictyostelium discoideum , activator (genetics) , stimulation , dictyostelium , cell division , propranolol , dna synthesis , chemistry , microbiology and biotechnology , slime mold , caffeine , cell , endocrinology , biology , biochemistry , receptor , dna , gene
Amoebae of the cellular slime mould Dictyostelium discoideum showed stimulated mitogenic activity when exposed to 200μM isoproterenol, an activator of adenyl cyclase, for 30min. Approximately 40% increase in cell proliferation was found at 48h after isoproterenol treatment. A faster and larger plaque formation as well as higher uptake of FITC‐labelled E. coli indicates greater phagocytotic activity in the treated cells. A concurrent increase in DNA and protein syntheses was also recorded in the treated cells. Administration of 400μM caffeine or 200μM (+) propranolol brought down the isoproterenol‐induced elevation in the cell division rate to control levels. These results are discussed in relation to a precocious activation of adenyl cyclase in the treated cells leading to a transient but significant increase in cell division in this organism.