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MINIPODIA AND ROSETTE CONTACTS ARE ADHESIVE ORGANELLES PRESENT IN FREE‐LIVING AMOEBAE
Author(s) -
Grbecki Andrzej,
Grbecka Lucyna,
Wasik Anna
Publication year - 2001
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.2001.0806
Subject(s) - rosette (schizont appearance) , amoeba (genus) , amoeba proteus , phalloidin , biophysics , adhesion , organelle , actin , staining , cytoskeleton , biology , chemistry , microbiology and biotechnology , materials science , cell , composite material , biochemistry , immunology , genetics
Using scanning electron microscopy, Amoeba proteus cells migrating on the glass have been shown to develop dense coats of minipodia, which are discrete microprotrusions up to 8μm long and ∼0.5μm across. They cover the middle‐anterior area of the ventral cell surface, i.e. the region previously determined as the zone of most efficient adhesion of an amoeba to its substratum. Minipodia are sparse underneath the frontal zone and lacking from the tail region. In amoebae that adhere to the glass without moving, have just started moving, or show unstable motor polarity, minipodia are grouped in rosette contacts, cauliflower‐like papillae composed of supporting platforms with crowns of minipodia emerging from them. Both structures abound with cytoskeletal F‐actin, as shown by staining with fluorescein‐conjugated phalloidin. Amoebae experimentally prevented from adhering to the substratum neither develop discrete minipodia nor rosette contacts.