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[Ca 2+ ] i RISE IN JURKAT E6‐1 CELL LINES FROM DIFFERENT SOURCES AS A RESPONSE TO 50Hz MAGNETIC FIELD EXPOSURE IS A REPRODUCIBLE EFFECT AND INDEPENDENT OF POLY‐ l ‐LYSINE TREATMENT
Author(s) -
Mattsson MatsOlof,
Lindström Ewa,
Still Maria,
Lindström Per,
Mild Hansson Kjell,
Lundgren Erik
Publication year - 2001
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.2001.0774
Subject(s) - jurkat cells , intracellular , calcium in biology , cell , calcium , lysine , endoplasmic reticulum , cell culture , peptide , chemistry , biophysics , microbiology and biotechnology , biology , biochemistry , t cell , immunology , genetics , immune system , organic chemistry , amino acid
Jurkat E6‐1 cells obtained from three different sources were compared with respect to intracellular calcium response to a 50Hz, 0.15mT, magnetic field, to treatment with poly‐ l ‐lysine and to protein expression at the cell surface. The fura‐2 single cell measurements were a replication study performed by three members of our group. The cells responded to the applied magnetic fields, although the percentage of responding cells was lower than in earlier studies. The geomagnetic field was backed off without changing the outcome of the intracellular calcium measurements. Fluorometric analyses showed no difference between the E6‐1 cells obtained from three sources with respect to the expression of cell surface marker molecules. The addition of the cell adhesive peptide, poly‐ l ‐lysine, did not itself cause any effects on the intracellular calcium concentration.