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MORPHOLOGICAL ALTERATION OF PERITONEAL MAST CELLS AND MACROPHAGES IN THE MOUSE PERITONEAL CAVITY DURING THE EARLY PHASES OF AN ALLERGIC INFLAMMATORY REACTION
Author(s) -
Oliani Sonia M.,
Lim Lina H. K.,
Christian Helen C.,
Pell Keith,
Das Anuk M.,
Perretti Mauro
Publication year - 2001
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.2001.0770
Subject(s) - degranulation , ovalbumin , peritoneal cavity , mast cell , chemistry , eosinophil , granule (geology) , compound 48/80 , ultrastructure , macrophage , immunology , pathology , biology , immune system , medicine , biochemistry , in vitro , anatomy , paleontology , receptor , asthma
We investigated the presence of mast cell granules in macrophages following an in vivo model of an allergic reaction. Injection of ovalbumin (100μg) into the peritoneal cavity of sensitised mice produced a rapid (within 2h) influx of neutrophils followed by a slower (after >4h) eosinophil migration. Ovalbumin treatment induced a high incidence (∼50%) of mast cell degranulation compared to control phosphated‐buffered saline‐treated mice. The majority (∼90%) of peritoneal macrophages contained mast cell granules as early as 2h post‐ovalbumin, with lower values at later time‐points, as determined by staining with Toluidine blue and Berberine sulphate. This was confirmed by electron microscopy which enabled us to identify the complex mast cell granule sub‐structural components in macrophage phagosomes. In conclusion, we used histochemical and ultrastructural analyses to show that mast cell granules become internalised with macrophages during the early stages of an experimental allergic reaction.