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RELEASE OF IL‐4 AND IL‐5 FROM HUMAN PERIPHERAL BLOOD MONONUCLEAR CELLS CO‐CULTURED WITH JAPANESE CEDAR POLLEN ANTIGEN IN VITRO
Author(s) -
Kimura Hirokazu,
Shibata Yukino,
Minakami Hisanori,
Hashizume Setsuko,
Oba Hiromi,
Sakaguchi Masahiro,
Otsuki Kunio,
Kozawa Kunihisa,
Kato Masahiko
Publication year - 2001
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.2001.0766
Subject(s) - peripheral blood mononuclear cell , antigen , immunology , in vitro , peripheral blood , interleukin , interleukin 2 , medicine , allergen , cytokine , allergy , chemistry , biochemistry
The Japanese cedar pollen (JCP) is a major allergen with respect to pollinosis in Japan. It is believed that interleukin‐4 (IL‐4) and interleukin‐5 (IL‐5) derived from lymphocytes and other cells play a pivotal role in allergic reactions. We investigated whether the JCP antigen stimulates the release of these cytokines by peripheral blood mononuclear cells (PBMCs). PBMCs from eight adults (five adults with JCP pollinosis and three adults without JCP pollinosis) were co‐incubated with purified JCP antigens. IL‐4 was released in response to JCP antigens in six of the eight subjects at 24h and in three subjects at 48h. IL‐4 release at 24h occurred in all five subjects with JCP pollinosis but in only one of the three subjects without pollinosis. IL‐5 was released in response to the JCP antigen in five of the eight subjects at 24h and 48h, including four of the five subjects with JCP pollinosis and one of the three subjects without pollinosis. These results suggest that PBMCs were more likely to release IL‐4 and IL‐5 in the presence of JCP pollinosis.