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STUDIES OF APOPTOSIS OF MALIGNANT LYMPHOMA CELLS INDUCED BY ARSENIC TRIOXIDE
Author(s) -
Zhang Y.,
Nie L.
Publication year - 2001
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.2001.0755
Subject(s) - apoptosis , arsenic trioxide , dna fragmentation , tunel assay , microbiology and biotechnology , flow cytometry , jurkat cells , fragmentation (computing) , chemistry , raji cell , lymphoma , biology , cancer research , programmed cell death , t cell , immunology , biochemistry , immune system , ecology
The present study investigated the effect of As 2 O 3 on malignant lymphoma cells. Cell apoptosis was detected by cell staining and TdT‐mediated dUTP Nick‐end Labelling (TUNEL). Cellular DNA and protein expression content were determined by immunohistochemistry and flow cytometry. It was found that 0.5–2.0μm /l As 2 O 3 could inhibit cell growth, including Raji cells and lymphoma cells from patients, and induce apoptosis, such as condensed chromatin and nuclear fragmentation with intact cell membrane, i.e. apoptotic body. It was also found that the cells of the sub‐G 1 phase increased significantly and bcl‐2 gene expression was greatly downregulated. However, this effect was not observed for Jurkat cells under the same conditions. We concluded that As 2 O 3 at a range of 0.5–2.0μm /l can inhibit the growth and induce apoptosis in malignant lymphoma cells, which may have therapeutic potential.