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cGMP‐REGULATED STORE‐OPERATED CALCIUM ENTRY IN HUMAN HEPATOMA CELLS
Author(s) -
Jiang J. L.,
Yu M. K.,
Chen Z. N.,
Chan H. C.
Publication year - 2001
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.2001.0751
Subject(s) - thapsigargin , extracellular , incubation , chemistry , calcium , cell culture , microbiology and biotechnology , biochemistry , biology , organic chemistry , genetics
This study aimed to investigate cGMP‐regulated store‐operated Ca 2+ entry in human 7721 hepatoma cells. [Ca 2+ ] i was measured using Fura2/AM. After incubation of the cells with 4μm thapsigargin, Ca 2+ entry was evoked by application of 1m m Ca 2+ to extracellular solution and was blocked by 3m m Ni 2+ , indicating the presence of store‐operated Ca 2+ entry in human 7721 hepatoma cell line. Application of 8‐Br‐cGMP reduced the [Ca 2+ ] i in hepatoma 7721 cells by 80%. These data demonstrated for the first time that store‐operated Ca 2+ entry pathway is present in human hepatoma cells, which is regulated by cGMP.