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ALTERNATIVE SPLICING OF RH BLOOD GROUP POLYPEPTIDE mRNA PRODUCES A NOVEL TRANSCRIPT CONTAINING A SHORT NUCLEOTIDE INSERTION ON HUMAN ERYTHROLEUKEMIC K562 CELLS
Author(s) -
Sakata Noriaki,
Yamazaki Kentaro,
Kogure Tadahisa,
Mukai Toshiji
Publication year - 2001
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.2001.0722
Subject(s) - exon , microbiology and biotechnology , biology , intron , genbank , genetics , rna splicing , gene isoform , complementary dna , alternative splicing , gene , nucleic acid sequence , stop codon , splice , insertion sequence , peptide sequence , rna , genome , transposable element
A novel isoform of the human Rh blood group polypeptide cDNA was isolated from human erythroleukemic K562 cells. This isoform was produced by deletion of the sequences derived from exons 2 and 3 of the RHCE gene and insertion of 44‐bp into the resulting junction between exon 1 and 4 derived sequence. The deduced amino acid sequence revealed that the 44‐bp insertion sequence contains an in‐frame stop codon that causes premature chain termination. A sequence homology search using GenBank showed that the inserted sequence was derived from the intron between exons 1 and 2 of the RHCE gene. Moreover, analysis of the region surrounding the inserted sequence indicated that the insert was a cryptic exon flanked by consensus donor and acceptor splice sequences. This novel transcript was most likely produced by alternative splicing.