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ASSESSMENT OF THE ANTITUMOUR ACTIVITY OF TARGETED IMMUNOSPECIFIC ALBUMIN MICROSPHERES LOADED WITH CISPLATIN AND 5‐FLUOROURACIL: TOXICITY AGAINST A RODENT OVARIAN CARCINOMA IN VITRO
Author(s) -
Truter Ernest J.,
Santos Aldina S.,
Els Willem J.
Publication year - 2001
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.2000.0677
Subject(s) - cisplatin , cytotoxicity , viability assay , toxicity , mtt assay , ovarian cancer , pharmacology , drug , ovarian carcinoma , in vitro , fluorouracil , chemistry , in vivo , biology , medicine , chemotherapy , cancer , biochemistry , microbiology and biotechnology
The 3‐(4,5 dimethylthiazol‐2‐yl)‐2,5 diphenyltetrazolium bromide (MTT) assay is used successfully to estimate the number of viable cells in drug screening trials. We used the MTT assay to assess the viability of a rodent ovarian carcinoma cell line (DMBA‐OC‐1R) after exposure to combinations of cisplatin and 5‐fluorouracil as free drug and in encapsulated (conjugated and unconjugated) forms. After 48h of exposure to free drugs, a significant trend towards cell cytotoxicity could be observed and this was well established by 120h. Cells treated with drug‐containing immuno‐microspheres showed a similar initial decrease in cell viability after 96h, and this was maintained for 128h. These results suggest that immuno‐microspheres loaded with chemotherapeutic drugs have the potential to be successfully used in the treatment of ovarian cancer.