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UTILIZATION OF AMINO ACIDS IN GROWING KIDNEY PROXIMAL TUBULE CELL CULTURES
Author(s) -
Ali Abdi Rashid,
Evans Peter J.
Publication year - 2001
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.2000.0667
Subject(s) - proximal tubule , kidney , renal tubule , tubule , chemistry , amino acid , microbiology and biotechnology , biochemistry , biology , endocrinology
The growth of rat kidney proximal tubule cells was monitored continuously by the cellular incorporation of [methyl‐ 14 C] thymidine using scintillating microplates. The radioisotope had no effect on cell proliferation over a 5 day period, neither was it extensively converted to thymine. Leibovitz L‐15 medium supplemented with bicarbonate proved a good growth medium and its high levels of carbohydrates and amino acids facilitated the appearance of intermediates in the cells' metabolism of additional radioactive amino acids. Kidney proximal tubule cells had a greater potential to process amino acids than BHK‐21 cells. The utilization of amino acids by proximal tubule cells differed from that of other organs. The amino acids could be classified into three classes. Members of the first type were only used for protein synthesis (arginine, lysine, histidine and tyrosine). The second class of amino acids yielded only one or two metabolites (leucine and isoleucine), while the last type gave more than two metabolites (alanine, aspartate, glycine, methionine, proline and valine).