Premium
CHARACTERIZATION OF MYOGENIC CELL MEMBRANE: SPONTANEOUS FORMATION OF HETEROKARYOTIC MYOTUBES BETWEEN TWO DIFFERENT KINDS OF MYOBLASTS
Author(s) -
Hirayama Etsuko,
Nakanishi Masami,
Kim Jeman
Publication year - 2001
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.2000.0665
Subject(s) - myogenesis , sendai virus , cell fusion , myocyte , heterokaryon , rous sarcoma virus , quail , microbiology and biotechnology , cell culture , biology , chemistry , virus , virology , mutant , genetics , endocrinology , gene
In a previous study, it has been shown that presumptive mouse C2 myoblast cells are strongly resistant to HVJ (hemaglutinating virus of Japan, Sendai virus)‐mediated cell fusion, but do become capable of fusion upon differentiation. Quail myoblasts transformed with a temperature‐sensitive mutant of Rous sarcoma virus (QM‐RSV cells) also become more sensitive to HVJ‐mediated cell fusion during differentiation. Investigations were undertaken to see whether heterokaryotic myotubes were formed spontaneously by co‐culture of two different kinds of myogenic cells, QM‐RSV cells and C2 cells. When both cells were committed to myotube formation, they spontaneously fused without HVJ on co‐culture. On the other hand, when both or one of the cells were in the presumptive state, heterokaryons were not formed by co‐culturing. Furthermore, committed QM‐RSV cells did not fuse with non‐myogenic cells. These results indicate that the membranes of myogenic cells change to become capable of fusion for myotube formation during differentiation.