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EARLY MEMBRANE DEPRESSIONS IN HEPATOCYTES CULTURED ON PRIMARIA™ SUPPORTS
Author(s) -
Griffiths B.J. Nathan,
Evans Peter J.
Publication year - 2001
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.2000.0623
Subject(s) - nucleolus , tubule , proximal tubule , hepatocyte , microbiology and biotechnology , cell , chemistry , electron microscope , morphology (biology) , membrane , cell membrane , phenotype , biophysics , peripheral blood mononuclear cell , kidney , biology , cytoplasm , in vitro , biochemistry , endocrinology , gene , genetics , physics , optics
The topography of spreading hepatocytes on positively charged Primaria™ plates was examined using scanning electron microscopy. The cells acquired a uniform morphology within 2h. The spreading was rapid and the surface of the cells showed early prominent depressions or dips. The hepatocytes had either one or two of these structures which corresponded to the frequency of mononuclear and binuclear cells, respectively. The nuclear origin of the dips was strengthened after 6h. They contained solid structures, whose number, size and shape were the same as nucleoli. The membrane dipping was independent of cell density and took place under conditions where phenotypic changes can occur. Kidney proximal tubule cells had no dips. Co‐cultures of hepatocytes and kidney proximal tubule cells showed that the cell types behave differently.