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PROTEIN KINASE C IN ERYTHROLEUKAEMIA CELLS: TEMPORAL VARIATIONS IN THE EXPRESSION OF THE ALPHA, EPSILON AND ZETA ISOFORMS
Author(s) -
Hammond K. D.,
Savage N.,
Littlewood M.
Publication year - 2000
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.2000.0569
Subject(s) - gene isoform , protein kinase c , messenger rna , biology , microbiology and biotechnology , alpha (finance) , protein kinase a , kinase , enzyme , gene , biochemistry , medicine , construct validity , nursing , patient satisfaction
Determinations of mRNA and enzyme protein analyses for the protein kinase C (PKC) isoforms, alpha, epsilon and zeta, representing the classical, novel and atypical groups, respectively, in murine erythroleukaemic (MEL) cells revealed the occurrence of cyclic behaviour in expression. Such rhythmicity was not apparent when multiple analyses using only a single time‐point were considered; the importance of incorporating a time component into experimental design was demonstrated. On induction of differentiation over a period of five days with hexamethylene bisacetamide, changes in the patterns of expression of mRNA and protein were apparent, as compared with those of untreated, proliferating cells. Modulation of the dynamics of the various PKC isoforms may represent a regulatory mechanism for the control of a diversity of cellular functions.