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INFLUENCE OF MEMBRANE FLUIDITY MODIFIERS ON LYSOSOMAL OSMOTIC SENSITIVITY
Author(s) -
Yang Lu,
Zhang GuoJiang,
Zhong YiGang,
Zheng YanZhen
Publication year - 2000
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.2000.0552
Subject(s) - membrane fluidity , chemistry , biophysics , membrane , lysis , fluorescence anisotropy , osmotic pressure , tonicity , biochemistry , cytolysis , osmotic shock , chromatography , biology , in vitro , cytotoxicity , gene
Since lysosomes are prone to osmotic lysis, we have examined the correlation between their physical state and sensitivity to osmotic challenge, using agents which modify membrane fluidity. The latency loss of β‐hexosaminidase after an incubation in hypotonic sucrose medium was followed under different conditions of membrane fluidity, recorded by steady‐state fluorescence anisotropy of 1,6‐diphenyl‐1,3,5‐hexatriene. Increasing fluidity of the lysosomal membranes with benzyl alcohol (BA) and greater rigidity caused by cholesteryl hemisuccinate (CHS) increased and decreased the enzyme latency loss, respectively. The effects of BA and CHS treatments on osmotic sensitivity were reversible subsequently by reciprocal treatments of the lysosomes with CHS and BA, respectively. The results indicate that the physical state of the membrane does indeed affect lysosomal osmotic stability.