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PRESENCE, UPTAKE AND LOCALIZATION OF AN IMMUNOREACTIVELY INTERLEUKIN 6 (IL‐6)‐LIKE MOLECULE IN TETRAHYMENA PYRIFORMIS
Author(s) -
Kõhidai László,
Kovács Péter,
LázárMolnár Eszter,
Csaba György
Publication year - 2000
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.2000.0538
Subject(s) - tetrahymena , confocal microscopy , tetrahymena pyriformis , biology , interleukin , microbiology and biotechnology , labelling , in vitro , confocal , cell culture , biochemistry , cytokine , immunology , genetics , geometry , mathematics
The unicellular Tetrahymena and its medium contain immunoreactively interleukin 6 (IL‐6)‐like molecules (hereinafter IL‐6) in a measurable quantity in the 24 h‐old cultures. This protozoan takes up exogenously supplied IL‐6 very quickly, and this can be found in similar amounts in both the cells and the media after 1h. After 24h (48h cultures), an equal amount of IL‐6 is present in the control and IL‐6‐treated cells and their media. By 120h, cells which have not had their medium changed retained the same quantity of IL‐6 as the control; however less than half was found in IL‐6‐treated cells. In the medium of 120h‐old cultures, there was a reduction of IL‐6 content relative to the 24h content in the control; however, in the IL‐6‐treated cell culture medium, less than half of the level in the controls was found. Confocal microscopy demonstrated the localization of IL‐6 in/on the oral apparatus and basal bodies, and the nuclear envelope also showed moderate labelling. IL‐6 antibody binding was enhanced after IL‐6 pretreatment (hormonal imprinting). The experiments call attention to the presence of an IL‐6‐like molecule and its uptake at a very low level of phylogeny.