Premium
SPECTRIN‐LIKE PROTEINS IN PLANT NUCLEI
Author(s) -
Ruijter Norbert C. A.,
Ketelaar Tijs,
Blumenthal Sonal S. D.,
Emons Anne Mie C.,
Schel Jan H. N.
Publication year - 2000
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.2000.0508
Subject(s) - spectrin , nucleolus , nucleoplasm , biology , cell nucleus , band 3 , microbiology and biotechnology , nucleus , biochemistry , cytoskeleton , membrane protein , cell , membrane
We analysed the presence and localization of spectrin‐like proteins in nuclei of various plant tissues, using several anti‐erythrocyte spectrin antibodies on isolated pea nuclei and nuclei in cells. Western blots of extracted purified pea nuclei show a cross‐reactive pair of bands at 220–240kDa, typical for human erythrocyte spectrin, and a prominent 60kDa band. Immunolocalization by means of confocal laser scanning microscopy reveals spectrin‐like proteins in distinct spots equally distributed in the nucleoplasm and over the nuclear periphery, independent of the origin of the anti‐spectrin antibodies used. In some nuclei tracks of spectrin‐like proteins are also observed. No signal is present in nucleoli. The amount and intensity of signal increases when nuclei were extracted, successively, with detergents, DNase I and RNase A, and high salt, indicating that the spectrin‐like protein is associated with the nuclear matrix. The labelling is similar in nuclei of various plant tissues. These data are the first that show the presence and localization of spectrin‐like epitopes in plant nuclei, where they may stabilize specific interchromatin domains.