KINETIC CHARACTERIZATION OF CALCIUM UPTAKE BY THE RAT LIVER GOLGI APPARATUS | Zendy

Rojas Patricio | Zendy; Surroca Amarilys | Zendy; Orellana Ariel | Zendy; Wolff Daniel | Zendy

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KINETIC CHARACTERIZATION OF CALCIUM UPTAKE BY THE RAT LIVER GOLGI APPARATUS

Author(s) -

Rojas Patricio,

Surroca Amarilys,

Orellana Ariel,

Wolff Daniel

Publication year - 2000

Publication title -

cell biology international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.932

H-Index - 77

eISSN - 1095-8355

pISSN - 1065-6995

DOI - 10.1006/cbir.2000.0496

Subject(s) - endoplasmic reticulum , thapsigargin , serca , golgi apparatus , vesicle , calcium , calmodulin , chemistry , calcium pump , atpase , biophysics , biochemistry , biology , enzyme , membrane , organic chemistry

We carried out a kinetic characterization of the Ca 2+ active transport in the rat liver Golgi Apparatus (GA) membrane. Calcium accumulation by vesicles of a GA enriched fraction was found to be a function of both Ca 2+ and ATP‐Mg concentrations, it was inhibited by 2μ m thapsigargin but not stimulated by 3μ m calmodulin. The kinetic parameter values obtained for the GA Ca 2+ pump were: J max of 3.96nmol/mg min, K m for Ca 2+ of 0.150μ m and two K m 's for ATP of 1.14μ m and 519μ m . These results were almost identical to those obtained for the endoplasmic reticulum (ER) fraction, indicating that the GA Ca 2+ pump is a sarco/endoplasmic reticulum (SERCA) P‐type, analogous—if not identical—to that present in the ER.

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