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GANGLIOSIDE‐MEDIATED METABOLIC SYNCHRONIZATION OF PROTEIN SYNTHESIS ACTIVITY IN CULTURED HEPATOCYTES
Author(s) -
Brodsky Vsevolod Y.,
Nechaeva Natalia V.,
Zvezditalia D.,
Novikova Tatjana E.,
Gvasava Inna G.,
Fateeva Valentina I.,
Prokazova Nina V.,
Golovanova Natalia K.
Publication year - 2000
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1999.0505
Subject(s) - ganglioside , polyclonal antibodies , hepatocyte , biochemistry , biology , incubation , antibody , cell culture , microbiology and biotechnology , in vitro , immunology , genetics
An ultradian oscillation of protein synthesis was detected by synchronization of metabolic activity in rat hepatocyte cultures. This oscillation occurs in dense cultures in fresh medium, but not in sparse ones. Metabolic synchronization of sparse cultures, however, was initiated by conditioned medium or addition of 0.3–0.5μ m of a mixture of bovine brain gangliosides to fresh culture medium along with either 0.06–0.2μ m GM1 or 0.1–0.2μ m GDIa. GTIb and GDIb did not produce oscillations, nor did human liver ganglioside GM3. High expression of GM1 ganglioside determinants in hepatocytes maintained in the conditioned medium purified polyclonal antibodies to GM1 was coupled with protein synthetic oscillatory activity, i.e. metabolic synchronization. Incubation of dense cultures with GM1‐antibodies for 24h decreased the amplitude of these oscillations. In sparse cultures maintained in fresh medium where protein synthesis showed no oscillatory pattern, GM1 expression was low.