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EFFECT OF SERUM WITHDRAWAL ON THE PROLIFERATION OF B16F10 MELANOMA CELLS
Author(s) -
RodriguezAyerbe Clara,
SmithZubiaga Isabel
Publication year - 2000
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1999.0502
Subject(s) - cell cycle , cell growth , dna synthesis , proliferating cell nuclear antigen , cell , microbiology and biotechnology , viability assay , biology , chemistry , andrology , dna , medicine , biochemistry
Abstract B16F10 murine melanoma cell proliferation was inhibited after 48h in medium with serum in the range 0.1 to 0.5% by volume. Cell viability was mostly retained, whereas cells completely deprived of serum died. Growth‐arrested cultures showed serum‐dependent suppression of DNA synthesis. The response was typically that of a ‘cell cycle freeze’, verified by flow cytometric distribution of cells. Consequently, serum deprivation did not lead to synchony when serum was restored to arrested populations. Furthermore, there was no change in PCNA expression in arrested cells.