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HISTOCHEMICAL CHARACTERIZATION OF CELL DEATH IN HONEYBEE LARVAE MIDGUT AFTER TREATMENT WITH PAENIBACILLUS LARVAE , AMITRAZ AND OXYTETRACYCLINE
Author(s) -
Gregorc Aleš,
Bowen Ivor D.
Publication year - 2000
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1999.0490
Subject(s) - midgut , biology , programmed cell death , microbiology and biotechnology , oxytetracycline , amitraz , american foulbrood , necrosis , apoptosis , dna fragmentation , larva , acaricide , antibiotics , botany , toxicology , biochemistry , genetics , spore
A number of techniques were employed to assess cell death induced in honeybee larvae midgut after per os inoculation of bacterium Paenibacillus larvae var. larvae , the causative agent of American foulbrood disease, and separately with acaricide Amitraz and antibiotic Oxytetracycline. In honeybee larvae exposed to Amitraz, which demonstrates both necrosis and apoptosis, cell death was found in 82% of midgut columnar and in 50% of regenerative epithelial cells, 24h after treatment. Cell death reduced to 36% in the epithelial cells, 48h after treatment. In Oxytetracycline‐treated larvae, cell death was identified in 40% of midgut epithelial cells, 24h after inoculation and increased to 55% over the next 24h. In Paenibacillus ‐infected larvae, all midgut epithelial cells died. Using ApopTag (Oncor) to label the multiple DNA ends generated by DNA fragmentation showed programmed cell death in 49% of columnar midgut cells 24h after Amitraz application. Cell death was reduced to 9% over the next 24h. Our data indicate that cell death could be identified and quantified in situ , using TUNEL techniques. This study also shows that the acaricide Amitraz is a trigger for programmed cell death in the midgut epithelial cells of honeybee larvae, unlike Paenibacillus which induces necrosis only. The data show that immunohistochemical methods are useful for studying in situ tissue pathology, and indicate possibilities for monitoring the effects of infective and chemical environmental stressors on cell death in honeybee larvae tissue.