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ARREST IN METAPHASE AND ANATOMY OF MITOTIC CATASTROPHE: MILD HEAT SHOCK IN TWO HUMAN OSTEOSARCOMA CELL LINES
Author(s) -
Erenpreisa Je,
Ivanov A.,
Dekena G.,
Vitina A.,
Krampe R.,
Freivalds T.,
Selivanova G.,
Roach H.I
Publication year - 2000
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1999.0466
Subject(s) - metaphase , mitosis , interphase , mitotic index , microbiology and biotechnology , biology , mitotic catastrophe , cell cycle checkpoint , cell cycle , apoptosis , programmed cell death , genetics , chromosome , gene
The exits from metaphase arrest and anatomy of mitotic catastrophe were studied in two human osteosarcoma cell lines, nontumorigenic HOS TE85 and its chemically transformed strain MNNG‐HOS, applying mild genotoxic damage by heat shock at 41.8°C for 24h. Under these conditions, both cell lines doubled or tripled their mitotic index entering arrest in metaphase. On return to 37°C, the arrest was either released or ended in apoptosis. The transformed strain showed a greater capacity to arrest in metaphase as well as a greater probability of developing the third pathway: to restitute this arrest in polyploid interphase. This, in turn, either entered an ‘endocycle’ or, following a delay, apoptosis. Thus, arrest in metaphase was a cross‐point of the mitotic cycle, apoptosis, and endocycle. Mitotic catastrophe can morphologically manifest combinations of elements of these three processes.