Premium
NEUTRAL ENDOPEPTIDASE‐24.11 (NEP) DEACTIVATES PDGF‐ AND TGF‐β‐INDUCED CELL SHAPE CHANGES IN INVERTEBRATE IMMUNOCYTES
Author(s) -
Caselgrandi Eva,
Kletsas Dimitris,
Ottaviani Enzo
Publication year - 2000
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1999.0453
Subject(s) - platelet derived growth factor receptor , mytilus , neprilysin , endopeptidase , microbiology and biotechnology , biology , proteolysis , transforming growth factor , chemistry , growth factor , biochemistry , enzyme , receptor , ecology
Using a cytofluorimetric assay, we found that immunocytes of the mollusc, Mytilus galloprovincialis , express CD10, a surface antigen known to be identical to neutral endopeptidase‐24.11 (NEP). The spectrofluorimetric analysis demonstrates that the growth factors PDGF‐AB and TGF‐β1 provoke an increase in NEP‐like activity in membrane preparations from the immunocytes, but have no effect on the soluble form in the serum. On the other hand, computer‐assisted microscopic image analysis reveals that NEP deactivates the PDGF‐AB‐ and TGF‐β1‐induced shape changes in immunocytes. However, Western blots show that, in solution, NEP does not cleave PDGF‐AB or TGF‐β1, indicating that the inactivation is not due to proteolysis. These results suggest a functional interplay in invertebrate immunocytes between growth factors and NEP, as previously shown in vertebrate cells.