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INHIBITION OF EARLY DNA‐DAMAGE AND CHROMOSOMAL ABERRATIONS BY TRIANTHEMA PORTULACASTRUM L. IN CARBON TETRACHLORIDE‐INDUCED MOUSE LIVER DAMAGE
Author(s) -
Sarkar Alok,
Pradhan Soumen,
Mukhopadhyay Indranil,
Bose Subrata K.,
Roy Shyamal,
Chatterjee Malay
Publication year - 1999
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1999.0439
Subject(s) - carbon tetrachloride , dna damage , ccl4 , dna , basal (medicine) , sugar , pharmacology , liver injury , biology , chemistry , biochemistry , endocrinology , organic chemistry , insulin
The underlying molecular mechanisms of the antihepatotoxic activity of Trianthema portulacastrum by monitoring its effect on mouse liver DNA‐chain break, sugar‐base damage and chromosomal aberrations, during chronic or acute treatment with carbon tetrachloride (CCl 4 ) have been studied. Daily oral feeding with the ethanolic extract (150mg/kg basal diet, per os ) was given 2 weeks before CCl 4 treatment and continued until the end of the experiment (13 weeks). T. portulacastrum extract offer unique protection ( P< 0.05–0.001) against the induction of liver‐specific structural‐type chromosomal anomalies 15, 30 or 45 days after the last CCl 4 insult, compared to control mice. This was further evidenced by extract‐mediated protection (15 days prior feeding following a single necrogenic dose of CCl 4 ) of the generation of DNA chain‐break and Fe‐sugar‐base damage assays. The observed hepatoprotective mechanism could be due to its ability to counteract oxidative injury to DNA in the liver of mouse.