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CELL FRACTIONATION ANALYSIS OF HUMAN CD8 GLYCOPROTEIN TRANSPORT BETWEEN ENDOPLASMIC RETICULUM, INTERMEDIATE COMPARTMENT AND GOLGI COMPLEX IN TISSUE CULTURED CELLS
Author(s) -
Erra Maria Carmen,
Iodice Luisa,
Lotti Lavinia Vittoria,
Bonatti Stefano
Publication year - 1999
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1999.0420
Subject(s) - golgi apparatus , endoplasmic reticulum , compartment (ship) , microbiology and biotechnology , cellular compartment , glycoprotein , secretory pathway , cell fractionation , stim1 , biology , glycosylation , cell , chemistry , biochemistry , membrane , oceanography , geology
We have set up an analytical cell fractionation procedure to dissect, by a non‐morphological method, the anterograde transport of proteins from endoplasmic reticulum, intermediate compartment and Golgi complex in tissue cultured cells. Using this procedure after pulse‐chase labelling of cells expressing human CD8 glycoprotein, we obtained results that: (1) support the view that the intermediate compartment is a distinct station in the export from the endoplasmic reticulum to the Golgi complex; and (2) strongly suggests that the O ‐glycosylation process starts after the intermediate compartment, presumably in the cis ‐Golgi complex.