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GENDER‐RELATED DIFFERENCES IN ADHESION, GROWTH AND DIFFERENTIATION OF VASCULAR SMOOTH MUSCLE CELLS ARE ENHANCED IN SERUM‐DEPRIVED CULTURES
Author(s) -
Bačáková L.,
Mareš V.,
Lisá V.
Publication year - 1999
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1999.0417
Subject(s) - microfilament , doubling time , actin , biology , vascular smooth muscle , smooth muscle , andrology , microbiology and biotechnology , cell , endocrinology , medicine , anatomy , cytoskeleton , biochemistry
Abstract In 1‐day cultures with 10% serum, the number of rat aortic smooth muscle cells (VSMC) adhering to the growth support was similar in cells from both sexes, whereas in 1% serum, the number of VSMC from male donors was lower. In 10% serum medium, the doubling time was significantly shorter and the number of [ 3 H]thymidine‐labelled nuclei was higher in cells of high passage from male rats. In serum‐free medium, these differences increased and were also seen in cells of low passage number. Morphologically, the cells in male‐derived cultures at higher passage number were mainly spindle‐shaped, formed well‐developed ‘hills and valleys’ and possessed longitudinally oriented bundles of α‐actin‐containing microfilaments. Most cells from female rats were flat, polygonal, the multilayered ‘hills’ were less prominent, with α‐actin microfilaments forming a mesh‐like network.