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EFFECTS OF 1α,25‐DIHYDROXYCHOLECALCIFEROL AND CORTISOL ON THE GROWTH AND DIFFERENTIATION OF PRIMARY CULTURES OF MOUSE MAMMARY EPITHELIAL CELLS IN COLLAGEN GEL
Author(s) -
Kanazawa Takuya,
Enami Jumpei,
Kohmoto Kaoru
Publication year - 1999
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1999.0406
Subject(s) - endocrinology , medicine , cell culture , cholera toxin , prolactin , cell growth , glucocorticoid , biology , chemistry , hormone , biochemistry , genetics
We examined the effects of 1α,25‐dihydroxycholecalciferol (1,25‐DHCC) and the glucocorticoid, cortisol, on primary mouse mammary epithelial cells in collagen gel cell culture systems. Physiological low concentrations (10 −11 –10 −9 m ) of 1,25‐DHCC stimulated growth of the cells in a collagen gel matrix culture in serum‐free DMEM+Ham's F12 (1:1) medium containing BSA, EGF and cholera toxin, and the cell number reached 1.8‐fold the control after 6d in culture. In contrast, supraphysiological concentrations (10 −8 –10 −7 m ) of 1,25‐DHCC suppressed cell growth. Cortisol produced similar, but smaller, dose‐dependent effects. The addition of serum to the culture medium masked the stimulatory effect of 1,25‐DHCC and both the stimulatory and inhibitory effects of cortisol. 1,25‐DHCC also affected casein synthesis by cells cultured in a serum‐free floating collagen gel culture containing prolactin, insulin and cortisol, enhancing synthesis at low concentrations (10 −11 –10 −9 m ) and inhibiting it above 10 −8 m . In the absence of cortisol, no detectable change in casein synthesis was induced by 1,25‐DHCC. These results suggest a physiological role for 1,25‐DHCC in stimulating both growth and differentiation of mouse mammary epithelial cells, though 1,25‐DHCC does not substitute for glucocorticoids in the differentiation of the cells.