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CALCIUM LEVELS DURING CELL CYCLE CORRELATE WITH CELL FATE OF DICTYOSTELIUM DISCOIDEUM
Author(s) -
Saran Shweta
Publication year - 1999
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1999.0379
Subject(s) - dictyostelium discoideum , cell cycle , intracellular , microbiology and biotechnology , calcium , calcium in biology , egta , cell , cell cycle progression , biology , biophysics , chemistry , biochemistry , organic chemistry , gene
Levels of intracellular calcium, (Ca 2+ ) i , from different stages of cell cycle of Dictyostelium discoideum were monitored using the fluorescent Ca 2+ ‐sensitive dye, Indo 1. Combinations of Ca 2+ ‐ionophore (A23187) and Ca 2+ ‐chelator (EGTA) resulted in the inhibition of progression of cell cycle. This delay was caused due to block in G 2 /M→S phase transition of the cell cycle. Rescue of the cell cycle progression was made with 0.5m m of exogenous Ca 2+ . High (Ca 2+ ) i levels overlapped with the S‐phase, of the cell cycle. Results indicate that a high (Ca 2+ ) i level during S‐phase is not required for cell cycle progression but for cell‐type choice mechanism at the onset of starvation, and these cells tend to follow the prestalk pathway.