Premium
Cisplatin inhibits folic acid chemotaxis and phagocytotic functions in dictyostelium discoideum
Author(s) -
Reddy T.B.K,
Chatterjee S.
Publication year - 1999
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1998.0335
Subject(s) - dictyostelium discoideum , chemotaxis , folic acid , cisplatin , chemistry , microbiology and biotechnology , dictyostelium , biochemistry , biology , chemotherapy , genetics , medicine , gene , receptor
Administration of 100 and 200μg/ml of cisplatin [ cis ‐diammine dichloro platinum (II)] for 1h to growing Dictyostelium discoideum cells severely affects folic acid chemotaxis and phagocytotic function in this organism. Following cisplatin treatment, cells show a much lower uptake of FITC labelled bacteria and a reduced plaque forming ability when plated on Eschericia coli seeded normal agar. Folic acid chemotaxis and folate deaminase activity are greatly inhibited in cisplatin‐treated Dictyostelium cells. SDS‐PAGE analysis shows a greater association of actin and myosin with the cell cortex of treated cells. These results have been discussed in relation to cisplatin's known ability to raise the levels of cytosolic calcium.