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PROTEIN KINASE C AFFECTS REFORMATION OF ENDOTHELIAL JUNCTIONS IN XENOPUS XTH‐2 CELLS
Author(s) -
Werner Nicole Susann,
Meyer Rainer,
Achenbach Friedhelm
Publication year - 1999
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1998.0318
Subject(s) - protein kinase c , vinculin , microbiology and biotechnology , cytoskeleton , xenopus , chemistry , actin , endothelial stem cell , cytoplasm , biophysics , kinase , phosphorylation , biology , focal adhesion , cell , biochemistry , in vitro , gene
Endothelial cells can reversibly be forced to suppress the formation of endothelial junctions (EJ) by cultivation in a low calcium medium. The authors localized vinculin and cadherin as marker proteins of EJ and actin as a cytoskeletal component by fluorescence microscopy, and used this cell model to study the reformation of endothelial junctions under conditions of activation and inhibition of protein kinase C (PKC). Inhibition of PKC by H‐7 leads to an acceleration of EJ reformation, while constitutive activation by TPA inhibits the reformation process.