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EFFECTS OF COLD ACCLIMATION ON THE ACTIVITY LEVELS OF CREATINE KINASE, LACTATE DEHYDROGENASE AND LACTATE DEHYDROGENASE ISOENZYMES IN VARIOUS TISSUES OF THE RAT
Author(s) -
Terblanche S. E.,
Masondo T. C.,
Nel W.
Publication year - 1998
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1998.0310
Subject(s) - lactate dehydrogenase , creatine kinase , isozyme , endocrinology , medicine , biology , acclimatization , adipose tissue , biochemistry , enzyme , botany
The effects of cold acclimation on the activity levels of creatine kinase, lactate dehydrogenase and lactate dehydrogenase isoenzymes in various tissues/ organs of the rat ( Rattus norvegicus ) were investigated. Male Sprague‐Dawley rats were divided into two groups. One group was housed at 4±1°C (experimental group) and the other at 24±1°C (control group) for six months. The rats were housed in single cages and had access to food and water ad libitum. The tissues/organs investigated were heart, liver, lung, kidney, gastrocnemius muscle and interscapular brown adipose tissue as well as serum. With the exception of lung, (which showed a decrease of 24%) total creatine kinase activity levels were significantly increased ( P< 0.05) in all the tissues/organs investigated (17–51%) as well as serum (34%), in cold acclimated animals. Cold acclimation also resulted in significantly increased ( P< 0.05) activity levels of lactate dehydrogenase in all the tissues/organs investigated (14–24%) as well as serum (35%). Cold exposure resulted in an increase of the activity levels of all the detectable isoenzymes of lactate dehydrogenase, although not always significant, in all the tissues/organs investigated as well as serum. The M 4 tetramer of lactate dehydrogenase was the only detectable isoenzyme in serum.