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GROWTH OF MDA‐MB‐231 CELL LINE: DIFFERENT EFFECTS OF TGF‐β 1 , EGF AND ESTRADIOL DEPENDING ON THE LENGTH OF EXPOSURE
Author(s) -
Mur Cristina,
MartínezCarpio Pere A.,
FernándezMontolí María E.,
Ramon Josep M.,
Rosel Pilar,
Navarro Miguel A.
Publication year - 1998
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1998.0306
Subject(s) - transforming growth factor , cell culture , epidermal growth factor , cell growth , endocrinology , growth inhibition , transforming growth factor beta , medicine , growth factor , cell , beta (programming language) , biology , chemistry , microbiology and biotechnology , biochemistry , receptor , genetics , computer science , programming language
The human cell line MDA‐MB‐231 is a prototype for the study of hormone‐independent breast cancer. Modification of cell growth behaviour has been observed after treating these cells with growth factors. EGF is a typical stimulatory growth factor for many cell types, whereas transforming growth factor β 1 (TGF‐β 1 ) acts with inhibitory character. Here we observed cell growth inhibition after EGF as well as after TGF‐β 1 treatments. Nevertheless, in the 42‐h experiments, EGF‐treated cultures grew before (18 hours) respect to the TGF‐β 1 and E 2 ‐treated cultures (24h), and in the 11‐day experiments, EGF‐treated cultures started growing (7 days) after TGF‐β 1 ‐treated cultures (5 days). Estradiol inhibited the proliferation of these cells only after several days of treatment.