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TWO‐DIMENSIONAL PROTEIN MAPS OF XENOPUS EGGS AND EMBRYOS AT DIFFERENT DEVELOPMENTAL STAGES
Author(s) -
TEDESCHI GABRIELLA,
BERNARDINI GIOVANNI,
MORTARINO MICHELE,
NEGRI ARMANDO,
CECILIANI FABRIZIO,
RONCHI SEVERINO
Publication year - 1998
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1998.0296
Subject(s) - xenopus , blot , embryo , polyacrylamide gel electrophoresis , biology , microbiology and biotechnology , gel electrophoresis , embryogenesis , genetics , biochemistry , gene , enzyme
Protein expression during the early development of Xenopus has been followed by 2D‐polyacrylamide gel electrophoresis (PAGE). The analysis of two‐dimensional maps of eggs and embryos at different stages of development has allowed the separation of more than 2000 spots. Identification of numerous polypeptides was obtained in four different ways: (1) immuno‐blotting; (2) amino terminal sequence after blotting on to PVDF membranes; (3) comigration; and (4) assignment in comparison with proteins separated by 2D techniques on reference maps such as human liver, red blood cells, plasma and cerebrospinal fluid reported in the Swiss 2D‐PAGE Data Base. The maps presented in this report are a step toward the study of the protein expression in Xenopus eggs and embryos and may be a powerful working tool since Xenopus embryos are popular models for the study of development.