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ALTERATIONS IN GLYCOSYLTRANSFERASES DURING MYELOID AND MONOCYTOID DIFFERENTIATION OF HL‐60 CELLS
Author(s) -
LIU AI HUA,
LIU FEI,
LI ZHONG,
GU JIAN XING,
CHEN HUI LI
Publication year - 1998
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1998.0293
Subject(s) - retinoic acid , chemistry , myeloid , myeloid leukemia , microbiology and biotechnology , cell culture , biochemistry , cellular differentiation , glycan , glycosyltransferase , phorbol , butyric acid , enzyme , biology , immunology , gene , glycoprotein , protein kinase c , genetics
HL‐60, a human promyelocytic leukemia cell line, can be differentiated to myeloid lineage by all‐ trans retinoic acid (ATRA), dimethylsulfoxide (DMSO) and n ‐butyric acid ( n ‐BA), or to monocytoid(monocytic/macrophagic) lineage by phorbol‐12‐myristate‐13‐acetate (PMA) and ganglioside GM 3 . The activity alterations of N ‐acetylglucosaminyltransferase III and V (GnT‐III, GnT‐V) as well as α‐1,6‐fucosyl‐tranferase (α1,6 Fuc T) were studied during the differentiation of HL‐60 cells by the above‐mentioned five inducers using the fluorescence (PA)‐labeled glycan‐HPLC method for GnT assays and biotin‐labeled glycan‐LCA affinity chromatography combined with the HRP‐avidin colorimetric method for α1,6 Fuc T assay. It was observed that after 3 days, all three enzymes decreased in HL‐60 cells induced by 1μmol/l ATRA and 0.6mmol/l n‐BA, while GnT‐III and α1,6 Fuc T increased, but GnT‐V still decreased after induction by 1% DMSO. GnT‐V and α1,6 Fuc T declined, while GnT‐III was elevated after induction by 0.1μmol/l PMA for 3 days. In contrast, GnT‐III increased after the treatment with 50μmol/l GM 3 for 3 or 6 days, but GnT‐V was not appreciably changed and α1,6 FucT was elevated after 6 days of GM 3 treatment. It may be concluded that the decrease of GnT‐V is the common change in myeloid differentiation and the increase of GnT‐III is the general alteration in monocytoid differentiation. The changes in the activities of glycosyltransferases were consistent with the structural changes in surface N ‐glycans previously found in our laboratory, i.e. that the antennary number of N ‐glycans decreased during myeloid differentiation by ATRA, and the amount of bisecting GlcNAc in N ‐glycans increased during monocytoid differentiation by PMA.