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TEMPORAL‐SPATIAL EXPRESSION OF TWO PARACENTROTUS LIVIDUS CELL SURFACE PROTEINS
Author(s) -
ROMANCINO DANIELE P.,
GHERSI GIULIO,
GAMBINO ROBERTO,
MONTANA GIOVANNA,
COSTA CATERINA,
CARLO MARTA
Publication year - 1998
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1998.0255
Subject(s) - blastula , embryo , gastrulation , mesenchyme , biology , cytoplasm , microbiology and biotechnology , embryogenesis , polarity in embryogenesis , embryonic stem cell , cell , anatomy , biochemistry , gene
The temporal expression of two cell surface proteins, called BEP1 and BEP4, during Paracentrosus lividus embryonic development was studied. These proteins are found in both monomeric and dimeric forms in egg and embryos and we have established that their specific form is related to their being in the cytoplasm or on the cell surface. The spatial distribution of BEP1 and BEP4 proteins in eggs and embryos was established by whole mount immunohistochemistry. These proteins are located in the animal part of unfertilized and fertilized eggs; thereafter they are much less represented in structures derived from the vegetal cells of the embryo such as the micromeres of the 16 cell stage, the primary mesenchyme of blastula and the gut of gastrula. At the prism stage BEP1 and BEP4 proteins are present to some ectodermal parts and thereafter, at the pluteus stage, to the oral region.