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A COMPARISON OF THE ANTI‐HISTONE AND APOP‐TAG TECHNIQUE FOR DEMONSTRATING APOPTOSIS WITH OPTION FOR SILVER ENHANCEMENT
Author(s) -
ALHAZZAA AMAL A.,
BOWEN I. D.,
BIRCHALL M. A.
Publication year - 1998
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1998.0228
Subject(s) - histone , dna fragmentation , apoptosis , dna , microbiology and biotechnology , fragmentation (computing) , formaldehyde , histone h3 , antibody , biology , chemistry , biochemistry , programmed cell death , immunology , ecology
A novel immunocytochemical method is presented for the qualitative detection of DNA fragmentation in apoptosis. Anti‐histone antibody is employed to localize exposed nucleosomal histones (H1, H2a, H2b, H3 and H4) rather than tagging the cut ends of fragmenting DNA as in conventional technique. The method was tested on squamous cell carcinoma of the larynx routinely fixed in formaldehyde and embedded in paraffin wax and compared with results obtained employing Apop‐Tag kit (Oncor).