Premium
INTERCELLULAR COMMUNICATION IN RAPIDLY PROLIFERATING AND DIFFERENTIATED C6 GLIOMA CELLS IN CULTURE
Author(s) -
GHOSH PARAMITA,
SINGH UDAI N.
Publication year - 1997
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1997.0193
Subject(s) - microinjection , gap junction , intracellular , microbiology and biotechnology , biology , cell , immunofluorescence , cell culture , cell junction , membrane permeability , antibody , membrane , immunology , biochemistry , genetics
Glial cells in the brain are known to provide structural and functional supports to neurons. To sustain such a supportive role, they have developed cell‐to‐cell communicating gap junctional channels. The authors studied the effect of dbcAMP on gap junctional channels mediated communication in C6 cells, a rat glioma cell line. Quantitative assessment of coupled cells under microscope after microinjection of a fluorescent dye was taken as a measure of junctional permeability. An enhanced coupling between cells was observed following dbcAMP treatment and this elevated coupling was found to be dependent on the duration of exposure of cells to dbcAMP. The studies have focused on a subtle shift in the spatial organization of the functional channels to the processes of dbcAMP induced differentiated cells from the cell cytoplasms and membranes of dbcAMP untreated cells. Immunofluorescence study with affinity purified antibody against gap junction further confirmed the spatial distribution of gap junctional protein(s) in the processes and also showed an increase in the density of the protein at the intercellular spaces in dbcAMP induced differentiated C6 glioma cells.