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ROLB EXPRESSION PATTERN IN THE EARLY STAGES OF CARROT SOMATIC EMBRYOGENESIS
Author(s) -
COLA ALESSANDRA,
POMA ANNA,
SPANÒ LAURA
Publication year - 1997
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1997.0190
Subject(s) - somatic embryogenesis , daucus carota , biology , gene expression , agrobacterium , embryo , gene , reporter gene , somatic cell , embryogenesis , microbiology and biotechnology , genetics , botany , transgene
The pattern of expression of the rolB gene, derived from the T‐DNA of the plant pathogen Agrobacterium rhizogenes , has been investigated during the early stages of somatic embryo formation in suspension cultures of carrot ( Daucus carota L.). The reporter gene GUS ( Escherichia coli β ‐glucuronidase), under transcriptional control of full‐length rolB promoter region, has been utilized in order to evaluate both qualitative and quantitative variations in the expression pattern. Fluorimetric measurements point to the developmental regulation of the gene, while results from histochemical analysis indicate that the promoter of rolB is firstly activated in the central (core) region of the globular embryos.