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SOMATOSTATIN‐LIKE IMMUNOREACTIVE CELLS IN THE GROUND SQUIRREL RETINA
Author(s) -
LUGO NIDZA,
BLANCO ROSA E.
Publication year - 1997
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1997.0170
Subject(s) - parasol cell , ganglion cell layer , retina , inner nuclear layer , ganglion , giant retinal ganglion cells , bistratified cell , population , biology , anatomy , immunostaining , intrinsically photosensitive retinal ganglion cells , immunocytochemistry , pathology , microbiology and biotechnology , immunohistochemistry , neuroscience , endocrinology , retinal ganglion cell , medicine , immunology , environmental health
Immunocytochemical techniques were employed to locate somatostatin (SS)‐containing cells in the retina of the 13‐lined ground squirrel ( Spermophilus tridecemlineatus ). In normal retinas immunostain was limited to neuronal processes, yet distinctly labeled somata were detected in retinas of animals pretreated with colchicine. Labeled cell bodies were located in the outermost and innermost portions of the inner nuclear layer (INL) and in the ganglion cell layer (GCL). The largest population of SS‐like immunoreactive neurons was found in the innermost INL. These cells were identified as small and medium sized amacrine cells whose soma diameters ranged from 4 to 14μm. A smaller population of immunoreactive cells was observed in the outermost region of the INL. These cells, presumptive horizontal cells, were found mainly in peripheral regions of the retina. Immunoreactive cells in the GCL were of two types: displaced amacrines, and retinal ganglion cells. SS‐positive axons in the optic fiber layer suggest that some of the immunoreactive GCL neurons were ganglion cells, and it is our opinion that these cells belong to a class of associational ganglion cells previously identified in other species.