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IL‐1 MODIFIES SYSTEM A TRANSPORT ACTIVITY IN HUMAN RHEUMATOID SYNOVIAL CELLS
Author(s) -
MAIRE VALÉRIE,
HERNVANN ALAIN,
MARECHAL HERVÉ,
EKINDJIAN OHVANESSE G.,
AUSSEL CHRISTIAN
Publication year - 1997
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1997.0169
Subject(s) - stimulation , amino acid , cytokine , chemistry , protein biosynthesis , interleukin , rheumatoid arthritis , biochemistry , biology , medicine , endocrinology
Given the importance of interleukin‐1 in both rheumatic diseases and the modulation of cell metabolic activities, we studied the action of this cytokine on the neutral amino acid transport A system on rheumatoid synovial cells. In these cells IL‐1 (1ng/ml) induced amino transport stimulation from 5min to 5h. This effect was obtained only after a starvation period. No concentration‐related effect was found for IL‐1‐stimulated MeAIB uptake, and the IL‐1‐mediated MeAIB uptake stimulation is independent of protein synthesis. Neosynthesis or post‐translational maturation of protein transport is a prerequisite for obtaining this effect. In conclusion, rheumatoid synovial cells exhibit a higher sensitivity for IL‐1 than osteoarthritic ones, probably related to their intense metabolic activity.