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PPRAS1 PPRAS2 AND PPRAP1 GENES, MEMBERS OF A RAS GENE FAMILY FROM THE TRUE SLIME MOLD PHYSARUM POLYCEPHALUM ARE DEVELOPMENTALLY REGULATED
Author(s) -
KOZLOWSKI PIOTR,
TRZCINSKADANIELEWICZ JOANNA,
URBANCZYK AGNIESZKA,
TOCZKO KAZIMIERZ
Publication year - 1997
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1997.0168
Subject(s) - physarum polycephalum , biology , multinucleate , slime mold , gene , gene family , mycetozoa , syncytium , gene expression , microbiology and biotechnology , genetics , cell , dictyostelium
The expression patterns of two true ras genes, Ppras1 and Ppras2 and one rap gene, Pprap1 were examined in four Physarum polycephalum developmental stages: uninucleate amoebae, plasmodia (multinucleate syncytia), spherules (a vegetative, dormant stage) and fruiting bodies. Ppras1 and Pprap1 are expressed in all stages examined with the maximum levels of their transcripts in amoebae and fruiting bodies, respectively, and the minimum levels in plasmodia, whereas the Ppras2 transcript is only detectable in amoebae and fruiting bodies. The results obtained indicate that P. polycephalum is an organism possessing a developmentally regulated ras gene family and presents a convenient system to study the role of ras / rap genes in control of growth and differentiation of lower eukaryotic organisms.