INFLUENCE OF SUCCESSIVE AND COMBINED ULTRAVIOLET A AND B IRRADIATIONS ON MATRIX METALLOELASTASES PRODUCED BY HUMAN DERMAL FIBROBLASTS IN CULTURE | Zendy

KUT C. | Zendy; HORNEBECK W. | Zendy; GROULT N. | Zendy; REDZINIACK G. | Zendy; GODEAU G. | Zendy; PELLAT B. | Zendy

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INFLUENCE OF SUCCESSIVE AND COMBINED ULTRAVIOLET A AND B IRRADIATIONS ON MATRIX METALLOELASTASES PRODUCED BY HUMAN DERMAL FIBROBLASTS IN CULTURE

Author(s) -

KUT C.,

HORNEBECK W.,

GROULT N.,

REDZINIACK G.,

GODEAU G.,

PELLAT B.

Publication year - 1997

Publication title -

cell biology international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.932

H-Index - 77

eISSN - 1095-8355

pISSN - 1065-6995

DOI - 10.1006/cbir.1997.0150

Subject(s) - ultraviolet b , chemistry , matrix (chemical analysis) , ultraviolet , materials science , dermatology , medicine , optoelectronics , chromatography

To study the cumulative influence of UV irradiations on skin matrix alterations, human skin fibroblasts were irradiated successively three‐fold, at 24h intervals, with UVA (3×5J/cm 2 ), UVB (3×8mJ/cm 2 ), UVA plus UVB (3×5J/cm 2 and 3×8mJ/cm 2 ) and the levels of 92kDa gelatinase (pro‐MMP 9 ), 72kDa gelatinase (pro‐MMP 2 ) and plasma‐membrane elastase type protease were determined, following subsequent 24‐h culture in 10% serum‐containing medium. UV irradiations had only minor influence (1.4‐fold increase for UVB) on secreted levels of pro‐MMP 2 and decreased the amount of plasma membrane elastase produced by cells. It did however, for UVA and UVB alone, induce a significant increase of 66kDa activated MMP 2 production: 2.5‐ and 1.7‐fold respectively. Such enhancement was not observed when combined irradiations were administered. UV exposure possessed a much higher influence on pro‐MMP 9 secretion by dermal fibroblast enhancing enzyme levels by 2.5‐, 6.5‐ and 5‐fold for UVA, UVB and UVA+UVB, respectively.

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