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VISUALIZATION OF GOLGI APPARATUS IN METHACRYLATE EMBEDDED CONIFER EMBRYO TISSUE USING THE MONOCLONAL ANTIBODY JIM 84
Author(s) -
EVANS DAVID E.,
CLAY PATRICIA J.,
ATTREE STEPHEN,
FOWKE LARRY C.
Publication year - 1997
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1997.0145
Subject(s) - immunogold labelling , immunocytochemistry , biology , hypocotyl , staining , monoclonal antibody , somatic embryogenesis , cotyledon , embryo , antigen , suspensor , electron microscope , golgi apparatus , botany , microbiology and biotechnology , anatomy , antibody , ultrastructure , embryogenesis , immunology , genetics , physics , endoplasmic reticulum , optics , endocrinology
Methacrylate embedding followed by resin removal has been used for the first time to visualize a membrane‐associated antigen at the tissue level. Monoclonal antibody JIM 84 was used to stain the Golgi apparatus of gymnosperm (conifer) embryos by light microscope immunocytochemistry. Specificity of labelling was confirmed by electron microscope immunocytochemistry using LR‐white resin. GA staining was evident in all stages of white spruce somatic embryo development from immature to mature. Some regions of the somatic embryos (e.g. root cap/suspensor region) stained more vigorously than other regions (hypocotyl/cotyledon end). GA also stained in roots of Monterey pine and Douglas fir. Unlike the situation in most angiosperms, JIM 84 antigen appears to be absent from the conifer plasma membrane. However, it appears to be present in representatives of both major classes of higher plants.