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STIMULATION OF COLLAGEN SYNTHESIS IN RAT CARDIAC FIBROBLASTS BY EXPOSURE TO HYPOXIC CULTURE CONDITIONS AND SUPPRESSION OF THE EFFECT BY NATRIURETIC PEPTIDES
Author(s) -
TAMAMORI MIMI,
ITO HIROSHI,
HIROE MICHIAKI,
MARUMO FUMIAKI,
HATA RYUICHIRO
Publication year - 1997
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1997.0130
Subject(s) - paracrine signalling , medicine , natriuretic peptide , stimulation , endocrinology , intracellular , in vivo , atrial natriuretic peptide , hypoxia (environmental) , cardiac fibrosis , npr2 , brain natriuretic peptide , npr1 , fibrosis , northern blot , chemistry , western blot , cardiac function curve , in vitro , type i collagen , messenger rna , biology , heart failure , receptor , biochemistry , gene , microbiology and biotechnology , organic chemistry , oxygen
Synthesis of type I and type III collagens by rat cardiac fibroblasts was stimulated when the cells were cultured under 95% N 2 /5% CO 2 for one hour followed by incubation under normoxic conditions for 24 hours. The stimulative effect was attenutated by the presence of atrial natriuretic peptide (ANP, 10 −6 m) or brain natriuretic peptide (BNP, 10 −6 m) in the culture medium. Northern blot analysis indicated that α1(I) and α1(III) collagen mRNA levels were also increased by hypoxia, and decreased with the addition of ANP or BNP in a dose‐dependent manner. These results indicate interaction between intracellular signals of a physical stimulus (hypoxic stress) and those of a chemical one (ANP or BNP) and demonstrate that both signals regulate collagen synthesis by cardiac fibroblasts at the levels of the mRNAs. The results also suggest that natriuretic peptides produced by cardiomyocytes in vivo may function as paracrine factors that play a role in the prevention of cardiac fibrosis in ischaemic heart diseases.